evaluation of in vitro production of ifn-γ, il-10, il-12 and il-13 by blood cells in patients with cutaneous leishmaniasis lesions

this study investigated the in vitro production of interferon-γ, interleukin (il)-10, il-12, and il-13, after antigenic stimulation of the cells (with leishmania antigen and lipopolysaccharide) using whole blood from patients with cutaneous leishmaniasis lesions caused by leishmania tropica and in volunteers with history of cutaneous leishmaniasis. elisa results showed that the mean production of interferon-γ by cells of whole blood in patients with lesions in response to leishmania antigen was significantly lower than corresponding values in volunteers with history of cutaneous leishmaniasis (p in this study, we have detected the decreased production of interferon- γ by cells of patients with lesions of cutaneous leishmaniasis in response to parasite antigen and unbalanced production of regulatory cytokines such as il-10 and il-13 using the whole-blood stimulation assay technique. the required small volume of blood and the rapid set up time are the advantages in this assay technique. using this assay for further immunodetection of cytokines may confirm its value for clinical investigation.